MUE For Chemical and Microbiological Analysis

Program Overview 

Measurement uncertainty describes a region about an observed value of a physical quantity which is likely to enclose the true value of that quantity. Reporting measurement uncertainty for chemical and microbiological analysis are encouraged in SAMM ISO/IEC 17025 accredited laboratories.

This training module is designed based on ISO Guide to the Expression of Uncertainty in Measurement, 1995 and Eurachem / CITAC Guide CG 4, Quantifying Uncertainty in Analytical Measurement, 2nd Edition and ISO/TS 19036 microbiology of food and animal feeding stuffs - guidelines for the estimation of measurement uncertainty for quantitative determination, are the current version that these laboratories are supposed to follow. Participants are guided through a series of hands on practice, conduct experiments, collect data and perform calculation, learning from the simplest example of expressing uncertainty to complex multiple unit. These models for example weighing of chemicals, dilution of reference materials and testing result base on mathematical formulae.

Examples are discussed so that participants can apply the knowledge in daily working environment, to design and modify uncertainty models. On top of these, the module does emphasis on its application which is beyond the content of the Guide. The course is practical, easy understanding and applicable to chemical and microbiological analysis.

Target group

Quality Managers, Technical Managers, Laboratory Managers, Supervisors, Chemist, microbiologist, Engineer, Signatories of test reports & certificates, Laboratory Personnel.

Learning Outcomes

At the end of the course, participants are expected to:

  • Understand the concept of measurement uncertainty and its application.
  • Apply the knowledge of measurement uncertainty in chemical and biological testing laboratories to meet the requirements of all ISO quality management systems.


Participants are required to complete a project usually at the end of training or submit within two weeks after the training

  • Certificate of competency - achieves score points above 70 %
  • Certificate of attendance - score points below 70 % or no submission of project.


Building life models calculations, case studies, brain storming, hands on practice, lecture, discussion, course project.

Course Content

Subjects to study

  • Introduction
  • Scope
  • Uncertainty relates to the use of instrument in the context of ISO Quality Management System
  • The Concept
  • Make a Model of the Measuring System
  • Identify all Sources of Uncertainty
  • Calculate the Standard Uncertainty u for each Parameter
  • Calculate the Combined Standard Uncertainty Uc
  • Calculate the Degree of Freedom
  • Calculate the Expanded Uncertainty
  • Reporting Uncertainty

Practical Activities

Model 1: Simple linear dimension measurement & estimation of uncertainty

  • Use Ishikawa chart to identify sources of uncertainty and build model equation
  • Convert sources of uncertainties to standard uncertainty
  • Combine standard uncertainties using equation No. 10 of the Guide
  • Determine effective degree of freedom using Welcsh Satterthwaite formula and value of K, convert factor using student t-table
  • Expand the combine standard uncertainty with K
  • This model enables the participant to understand the concept of measurement uncertainty evaluation

Model 2: Weighing of chemical and evaluate uncertainty of weighing. This model include the application of measurement uncertainty in the context of ISO QMS for measuring instrument fitness for use

Model 3: Two dimensions, single measurement unit e.g. calculate area, coefficient of sensitivity & estimate its uncertainty

Model 4: Dilute reference material and evaluate the measurement uncertainty adopted from model 3.

Model 5: Evaluate measurement uncertainty of chemical testing where the result derive from mathematical formula.


Download Course Details



Course Title

MUE - For Chemical and Microbiological Analysis L004-B



To be determined by customer.



2 days.



9.00am – 5.00pm.



Customer’s premises (In-House Program) / Ascendent's Training Room (Public Program).


Class Size

Min. 5 - Max. 25 participants.


HRDF Status

SBL full payment. Crossed check is to be payable to ASCENDENT TECHNOLOGY SDN BHD. Admittance will be permitted upon receipt of full payment 2 weeks before the course is conducted.


Terms and Conditions

Ascendent Consultancy reserves the right to cancel or postpone due to unforeseen circumstances. The course fee, less 50% for cancellation, will be promptly refunded for cancellations received in writing at least seven (7) working days before the course. No refunds shall be made after this period.